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3 plasmids (pGL1124, pGL1224 and pGL1217) were manufactured to allow the substitution of one allele of CYC9Lister 427 cells. Trypanosomes have been transfected with each plasmid individually, then subjected to the next transfection with among the other plasmids, with a distinct resistance gene, with all pairwise mixtures done.Blocking the conserved